Blood flow cytometry in Mugil cephalus and Carassius auratus: a comparative study

Vincenzo Parrino, Tiziana Capello, Gregorio Costa, Carmela Cannavà, Marilena Sanfilippo, Francesco Fazio, Giuseppe Piccione, Salvatore Fasulo

Abstract


Blood and its parameters can give specific indications on the welfare of fishes.Many endogenous and exogenous factors exert influences on the characteristics of blood. The correct interpretation of fish hematology for a given species depends on the availability of reference values. The purpose of the present study was therefore to build a database with data on haematological profiles of Mugil cephalus (Linnaeus 1758) and goldfish Carassius auratus (Linnaeus 1758), in particular of some blood cells, namely red blood cells (RBC), white blood cells (WBC) and thrombocytes (TC). All the blood parameters studied showed significant differences in the two fish species considered, using flow cytometry and optical microscopy coupled with an automated system. In particular, RBC showed an increase in mullets in respect to goldfish, while WBC and TC decreased. The differences found may be due to the environmental conditions and the different eating habits of the two species. The results of this research will allow to better understand how the different dietary habits and environmental conditions can influence the haematological parameters of fishes. Flow cytometry represents a modern diagnostic technique in human. Moreover, the technique used by combining flow cytometry with automated haematological counting, has proved very effective in the early evaluation of haematological parameters of various fish species.

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DOI: http://dx.doi.org/10.1478/AAPP.97S2A15

Copyright (c) 2019 Vincenzo Parrino

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