Identificazione di prodotti ittici mediante dna barcoding
Fish identification by dna barcoding

Puglisi, M. L. and Reale, S. and Giunta, R. and Salamanca, A. and Vitale, F. and Cosenza, M. and Alfonzetti, T. (2013) Identificazione di prodotti ittici mediante dna barcoding
Fish identification by dna barcoding. In: ATTI X CONGRESSO NAZIONALE SO.F.I.VET., 8 – 9 luglio 2013, Taormina (Messina). (Submitted)

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Abstract

ABSTRACT – The authors developed and evaluated DNA barcodes for use in differentiating domestic and
imported fish species (1). First, we sequenced part of the cytochrome oxidase I (COI) gene in accordance
with standard DNA barcoding protocols. These alignments allowed the development and analyses of
consensus barcode sequences for each species and comparison with limited sequences in public databases
(GenBank and Barcode of Life Data Systems). In this study, we report test regarding Mediterranean fish
identifications. Experimental validation parameters were obtained on certain specie-specific DNA and
sensitivity, specificity, reproducibility, concordance tests role out value as 100%, 100%, 99%, 99.8%
respectively. The advantage of using “CO1”gene is that it is short enough to be sequenced quickly and
cheaply yet long enough to identify variations among species, and enabling identifications where traditional
methods are not “applicable, such as for immature stages or body fragments (2). FISH-BOL will also provide
a powerful tool for enhanced understanding of the natural history and ecological interactions of fish species.

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Item Type:	Conference or Workshop Item (Poster)
Subjects:	M.U.S. - Miscellanea > Atti Convegno > SO.F.I.VET 2013 - X Congresso nazionale > Poster
Depositing User:	Dr V T
Date Deposited:	30 Jul 2013 11:22
Last Modified:	30 Jul 2013 11:22
URI:	http://cab.unime.it/mus/id/eprint/5125

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