Studio delle proprietà biologiche di una specie della flora spontanea siciliana: Matthiola incana (L.) R. Br. (Brassicaceae)

Barbalace, Roberta (2018) Studio delle proprietà biologiche di una specie della flora spontanea siciliana: Matthiola incana (L.) R. Br. (Brassicaceae). Laurea Magistrale thesis, UNSPECIFIED.

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Abstract

The spontaneous flora of Sicily (Italy) includes several species belonging to the Brassicaceae family, potential source of bioactive compounds. Matthiola incana (L.) R. Br. is a biennial, short-lived perennial or annual herb (10-80 cm) native to the Mediterranean region in southern and western Europe, North Africa and the Middle East. This species is commonly used for ornamental purposes, due to its colourful flowers, and has become an economically important floral crop. Literature data report the use of M. incana in the traditional medicine of different countries for the treatment of various ailments, including inflammations and cancer; nonetheless, very few researches have been performed to investigate the phytochemistry and the biological activities of M. incana aerial parts. Thus, the present work was undertaken to characterize the phenolic profile and to evaluate some biological activities of a hydroalcoholic extract (80% methanol) obtained from the aerial parts (leaves and flower buds) of M. incana grown wild around Capo D’Orlando (Messina, Sicily). The quali-quantitative characterization of the phenolic compounds contained in the extract was attained by HPLC-PDA-ESI-MS. In order to extensively characterise the antioxidant potential of M. incana extract, three “in vitro” systems based on fundamentally different approaches and mechanisms were utilized: the primary antioxidant activity was examined by DPPH and reducing power assays; the secondary antioxidant properties were determined by ferrous ions chelating activity assay. M. incana extract exhibited moderate radical scavenging activity in the DPPH test and mild reducing power, whereas it showed good chelating properties. In order to establish the antioxidant efficacy in a more complex biological setting, the ability to protect bacterial growth from the oxidative stress induced by hydrogen peroxide (H2O2) was evaluated on Escherichia coli. The extract promoted the growth of E. coli under oxidative stress. The antimicrobial potential of M. incana was evaluated by standard methods. The MIC values obtained from the antimicrobial assays indicated that the extract is not effective against the bacteria and yeast strains tested in the study. Finally, M. incana extract resulted non-toxic in the Artemia salina Leach (brine shrimp) lethality bioassay. The obtained results contribute to an increase in knowledge about this species, as well as demonstrating that the aerial parts of Matthiola incana are a safe source of natural antioxidant compounds.

Item Type: Tesi (Laurea Magistrale)
Subjects: Scienze Chimiche
Dipartimento: Dipartimento di Scienze chimiche, biologiche, farmaceutiche ed ambientali
Depositing User: Mrs Cettina Cosenza
Date Deposited: 24 Jan 2019 07:22
Last Modified: 24 Jan 2019 07:22
URI: http://cab.unime.it/tesi/id/eprint/3275

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