Effectiveness of thymus vulgaris essential oil on ovine mammary pustular dermatitis

Vincenzo Naccari, Bianca Maria Orlandella, Clara Naccari

Abstract


The effectiveness of Thymus vulgaris L. Essential Oil (TvEO) for topical use in 21 sheep affected by mammary pustular dermatitis (MPD), not subjected to previous antibacterial therapy, was studied. After the evaluation of the antimicrobial susceptibility of isolated microorganisms(Staphylococcus epidermidis and Staphylococcus aureus) in milk samples to TvEO, some antimicrobials drugs (amoxicillin-clavulanic acid, doxycycline, florfenicol and enrofloxacin) and Citrus bergamia Risso et Poiteau EO (CvEO), the sheep, divided in two groups, were treated topically for 7 days with TvEO(13 animals) and CvEO (8 animals) respectively. The results showed that only TvEO induced a decrease in clinical signs 3 days after treatment and the complete disappearance of udder lesions after 7 days. No revival of infection was observedin the following months. The use of TvEOcould be an alternative to antibiotic treatment in ovine affected by MPD


Keywords


Thymus vulgaris EO; Citrus bergamia EO; mammary pustular dermatitis; ovine

Full Text:

PDF

References


1. Fratini, F., Casella, S., Leonardi, M., Pisseri, F., Ebani, V.V., Pistelli, L., Pistelli, L. (2014). Antibacterial activity of essential oils, their blends and mixtures of their main constituents against some strains supporting livestock mastitis. Fitoterapia, 96, 1-7. doi:10.1016/j.fitote.2014.04.003.

2. Mondello, F., Girolamo, A., Di Vito, M. (2015). Potenzialità degli oli essenziali nelle malattie infettive. Essential oils for humans health and the environment. Rapp. ISTISAN 15/6, ii: 26- 31.

Kon, K.V. and Rai, M.K. (2012). Plant essential oils and their constituents in copying with multidrug-resistant bacteria. Expert Rev Anti Infect Ther, 10, 775-790. https://doi.org/10.1586/eri.12.57.

3. Savoia, D. (2012). Plant-derived antimicrobial compounds: alternatives to antibiotics. Future Microbiol, 7, 979-990. https://doi.org/10.2217/fmb.12.68.

4. Scozzoli, M. (2015). Possibili applicazioni degli oli essenziali in medicina veterinaria. In: Mondello, F., Marella, A.M., Bellardi, M.G. and De Vito, M., Eds., Essential oils for humans health and the environment. Rapp. ISTISAN 15/6, Vol. 2, 56-59.

5. Bassolè, I.H. and Juliani, H.R. (2012). Essential oils in combination and their antimicrobial properties. Molecules, 17, 3989-4006. https://doi.org/10.3390/molecules17043989.

6. Fisher, K. and Phillips, C.A. (2006). The effect of lemon, orange and Bergamot essential oils and their components on the survival of Campylobacter jejuni, Escherichia coli O157, Listeria monocytogenes, Bacillus cereus and Staphylococcus aureus in vitro and in food systems. J. Appl. Microbiol., 101, 1232-1240. https://doi.org/10.1111/j.1365- 2672.2006.03035.x.

7. Lang, G. and Buchbauer, G. (2012). A review on recent research results (2008/ 2010) on essential oils as antimicrobials and antifungals. Flavour Fragr J, 27, 13-19.doi: 10.1002/ffj.2082

Solorzano-Santos, F. and Miranda-Novales, M.G. (2012). Essential oils from aromatic herbs as antimicrobial agents. Curr. Opin. Biotechnol., 23, 136- 141. doi: 10.1016/j.copbio.2011.08.005

8. Warnke, P.H., Lott, A.J., Sherry, E., Wiltfang, J., Podschun, R. (2013). The ongoing battle against multi- resistant strains: In vitro inhibition of hospital-acquired MRSA, VRE, Pseudomonas, ESBL E. coli and Klebsiella species in the presence of plant- derived antiseptic oils. J Craniomaxillofac Surg, 41, 321-326.https://doi.org/10.1016/j.jcms.2012.10.012

9. Pinto, E., Pina-Vaz, C., Salgueiro, L., Gonçalves, M. J., Costa-de-Oliveira, S., Cavaleiro, C., Palmeira, A., Rodrigues, A., Martinez-de-Oliveira, J. (2006). Antifungal activity of the essential oil of Thymus pulegioides on Candida, Aspergillus and dermatophyte species. J Med Microbiol, 55, 1367- 1373. https://doi.org/10.1099/jmm.0.46443-0

10. Ribeiro Salgueiro, L., Pinto, E., Gonçalves, M. J., Costa, I., Palmeira, A., Cavaleiro, C., Pina‐Vaz, C., Gonçalves Rodrigues, A., Martinez‐de‐Oliveira, J. (2006). Antifungal activity of the essential Oils of Thymus capitellatus against Candida, Aspergillus and dermatophyte strains. Flavour Fragr J, 21, 749-753. https://doi.org/10.1002/ffj.1610

11. Soković, M.D, Vukojević, J., Marin, P.D., Brkić, D.D., Vajs, V., Van Griensven, L. J. L. D. (2009). Chemical composition of essential oils of Thymus and Mentha Species and their antifungal activities. Molecules, 14, 238-249.https://doi.org/10.3390/molecules14010238.

12. Martin, K. and Ernst, E. (2003). Antiviral agents from plants and herbs: A systematic Review. Antivir Ther, 8, 77-90. https://doi.org/10.1111/j.2042-7166.2003.tb05815.x.

13. Pisseri, F., Bertoli, A., Pistelli, L. (2008). Essential oils in medicine: principles of therapy. Parassitologia, 50, 89-91.

14. Campagna, P. (2015). Oli essenziali in Clinica Dermatologica. TMA, 2, 32-34.Pisseri, F. (2015). Oli essenziali in Dermatologia Veterinaria. Rapp. ISTISAN 15/6, ii: 59-62.

15. Pisseri, F. (2015). Oli essenziali in Dermatologia Veterinaria Rapp. ISTISAN 15/6, ii: 59-62.

16. Mugnaini, L., Nardoni, S., Pistelli, L., Leonardi, M., Giuliotti, L., Benvenuti, M.N., Pisseri, F., Mancianti, F. (2013). A herbal antifungal formulation of Thimus serpillum, Origanum vulgare and Rosmarinus officinalis for treating ovine dermatophytosis due to Thricophitum mentagrofites. Mycoses , 56, 333-337.https://doi.org/10.1111/myc.12034|.

17. Rota, M.C., Herrera, A., Martinez, R.M., Sotomayor, J. A., Jordán, M.J (2008). Antimicrobial activity and chemical composition of Thymus vulgaris, Thymus zygis and Thymus hyemalis essential oils. Food Control, 19, 681-687.doi:10.1016/j.foodcont.2007.07.007

18. Ferreira, L.E., Benincasa, B.I., Fachin, A.L., França, S.C., Contini, S.S.H.T., Chagasm, A.C.S., Beleboni, R.O. (2016). Thymus vulgaris L. essential oil and its main component thymol: Anthelmintic effects against Haemonchus contortus from sheep. Vet Parasitol. 228, 70-76.doi.org/10.1016/j.vetpar.2016.08.011.

19. Ksouri S., Djebir, S., Bentorki, A. A., Gouri, A., Hadef, Y., Benakhla, A. (2016). Antifungal activity of essential oils extract from Origanum floribundum Munby, Rosmarinus officinalis L. and Thymus ciliatus Desf. against Candida albicans isolated from bovine clinical mastitis. J Mycol Med. 27(2), 245-249. doi.org/10.1016/j.mycmed.2017.03.004.

20. Zhengkai, W., Ershunm, Z., Changming, G., Yunhe, F., Yuqiang, Y., Yimeng L., Minjun, Y., Naisheng, Z., Zhengtao, Y. (2014). Thymol inhibits Staphylococcus aureus internalization into bovine mammary epithelial cells by inhibiting NF-kB activation. Microb. Pathog 71-72, 15-19. doi.org10.1016/j.micpath.2014.01.004.

Naccari, V., Orlandella, B. M., Fisichella, V., Naccari, F., Caracappa S. (2017). Effectiveness of Thymus vulgaris essential oil in the treatment of skin infections in dogs. Open J Vet Med, 2017, 7, 63-75. ISSN Online:2165-3364. doi: 10.4236/ojvm.2017.76007




DOI: https://doi.org/10.6092/1828-6550/APMB.107.2.2019.OS3

Refbacks

  • There are currently no refbacks.


Copyright (c) 2019 Vincenzo Naccari, Bianca Maria Orlandella, Clara Naccari

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.